This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for HIRA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HIRA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HIRA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:19,144,380–19,144,950 | 287.0 kb | Distal (>10kb) Multiome | 696 | |
| chr22:19,149,463–19,150,252 | 282.1 kb | Distal (>10kb) Multiome | 173 | |
| chr22:19,169,265–19,169,982 | 262.0 kb | Distal (>10kb) Multiome | 285 | |
| chr22:19,170,964–19,172,196 | 260.1 kb | Distal (>10kb) Multiome | 819 | |
| chr22:19,177,562–19,180,087 | 252.9 kb | Distal (>10kb) Multiome | 875 | |
| chr22:19,290,875–19,292,389 | 139.9 kb | Distal (>10kb) Multiome | 854 | |
| chr22:19,431,488–19,432,799 | 634 bp | At TSS Multiome | 1140 | |
| chr22:19,446,962–19,448,379 | 15.9 kb | Distal (>10kb) Multiome | 889 | |
| chr22:19,478,898–19,480,311 | 47.9 kb | Distal (>10kb) Multiome | 867 | |
| chr22:19,523,289–19,524,793 | 91.9 kb | Distal (>10kb) Multiome | 242 | |
| chr22:19,717,840–19,718,731 | 286.5 kb | Distal (>10kb) Multiome | 592 | |
| chr22:19,723,413–19,724,584 | 292.5 kb | Distal (>10kb) Multiome | 572 | |
| chr22:19,730,516–19,731,296 | 299.2 kb | Distal (>10kb) Multiome | 743 |
Genomic view of the HIRA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.