HAND2

heart and neural crest derivatives expressed 2 | Hed, Thing2, bHLHa26, dHand

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development. [provided by RefSeq, Jul 2008]

Biological processes 61 terms

DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)E-box binding (GO:0070888)E-box binding (GO:0070888)E-box binding (GO:0070888)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)RNA polymerase II-specific DNA-binding transcription factor binding (GO:0061629)RNA polymerase II-specific DNA-binding transcription factor binding (GO:0061629)adult heart development (GO:0007512)cardiac neural crest cell development involved in outflow tract morphogenesis (GO:0061309)cardiac neural crest cell development involved in outflow tract morphogenesis (GO:0061309)cardiac right ventricle formation (GO:0003219)cellular response to retinoic acid (GO:0071300)chromatin (GO:0000785)embryonic skeletal system development (GO:0048706)heart development (GO:0007507)heart development (GO:0007507)heart development (GO:0007507)minor groove of adenine-thymine-rich DNA binding (GO:0003680)negative regulation of cardiac muscle cell apoptotic process (GO:0010667)negative regulation of cardiac muscle cell apoptotic process (GO:0010667)negative regulation of gene expression (GO:0010629)noradrenergic neuron differentiation (GO:0003357)nucleus (GO:0005634)nucleus (GO:0005634)outflow tract morphogenesis (GO:0003151)outflow tract morphogenesis (GO:0003151)positive regulation of ERK1 and ERK2 cascade (GO:0070374)positive regulation of cardiac muscle hypertrophy (GO:0010613)positive regulation of cardiac muscle hypertrophy (GO:0010613)positive regulation of gene expression (GO:0010628)positive regulation of p38MAPK cascade (GO:1900745)positive regulation of semaphorin-plexin signaling pathway (GO:2001262)positive regulation of semaphorin-plexin signaling pathway (GO:2001262)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)protein dimerization activity (GO:0046983)protein homodimerization activity (GO:0042803)protein-containing complex (GO:0032991)regulation of secondary heart field cardioblast proliferation (GO:0003266)regulation of secondary heart field cardioblast proliferation (GO:0003266)regulation of tissue remodeling (GO:0034103)regulation of transcription by RNA polymerase II (GO:0006357)sequence-specific DNA binding (GO:0043565)sequence-specific double-stranded DNA binding (GO:1990837)thymus development (GO:0048538)thymus development (GO:0048538)transcription cis-regulatory region binding (GO:0000976)transcription cis-regulatory region binding (GO:0000976)transcription coactivator binding (GO:0001223)transcription coactivator binding (GO:0001223)transcription regulator complex (GO:0005667)transcription regulator complex (GO:0005667)

Expression (TPM)

TFs regulating HAND2 0 TFs

Transcription factors with Perturb-seq knockdown data for HAND2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HAND2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to HAND2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HAND2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr4:173,518,462–173,519,114	9.8 kb	Proximal (<10kb)	140
chr4:173,521,529–173,524,247	4.7 kb	Proximal (<10kb)	413
chr4:173,524,610–173,525,886	3.0 kb	Proximal (<10kb)	224
chr4:173,526,721–173,528,011	900 bp	At TSS	247
chr4:173,528,309–173,531,024	at TSS	At TSS	426
chr4:173,531,197–173,532,084	2.3 kb	Proximal (<10kb)	195
chr4:173,536,826–173,537,384	7.9 kb	Proximal (<10kb)	119
chr4:173,537,707–173,539,114	8.8 kb	Proximal (<10kb)	380

Genome Browser

Genomic view of the HAND2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.