Transcription factors with Perturb-seq knockdown data for GUSBP5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GUSBP5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GUSBP5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:143,558,240–143,560,120 | at TSS | At TSS | 594 | |
| chr4:143,563,118–143,563,407 | 3.7 kb | Proximal (<10kb) | 6 | |
| chr4:143,699,056–143,701,357 | at TSS | At TSS | 329 | |
| chr4:144,078,497–144,079,298 | at TSS | At TSS | 143 |
Genomic view of the GUSBP5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.