The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]
Transcription factors with Perturb-seq knockdown data for GPX3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GPX3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GPX3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:150,758,281–150,759,438 | 261.7 kb | Distal (>10kb) Multiome | 902 | |
| chr5:150,778,240–150,779,492 | 241.6 kb | Distal (>10kb) Multiome | 723 | |
| chr5:150,783,348–150,783,999 | 236.9 kb | Distal (>10kb) Multiome | 435 | |
| chr5:150,801,105–150,801,901 | 219.1 kb | Distal (>10kb) Multiome | 382 | |
| chr5:151,002,371–151,003,402 | 17.7 kb | Distal (>10kb) Multiome | 132 | |
| chr5:151,020,249–151,021,200 | 50 bp | At TSS Multiome | 522 | |
| chr5:151,080,524–151,081,640 | 60.4 kb | Distal (>10kb) Multiome | 916 | |
| chr5:151,157,085–151,158,664 | 137.2 kb | Distal (>10kb) Multiome | 760 | |
| chr5:151,223,077–151,224,282 | 203.4 kb | Distal (>10kb) Multiome | 662 | |
| chr5:151,252,199–151,253,942 | 232.4 kb | Distal (>10kb) Multiome | 746 |
Genomic view of the GPX3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.