The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GPA33. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GPA33 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GPA33, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:166,875,665–166,876,831 | 290.1 kb | Distal (>10kb) Multiome | 947 | |
| chr1:166,883,947–166,884,647 | 282.0 kb | Distal (>10kb) Multiome | 218 | |
| chr1:166,920,313–166,921,576 | 245.5 kb | Distal (>10kb) Multiome | 206 | |
| chr1:166,975,212–166,976,080 | 190.9 kb | Distal (>10kb) Multiome | 470 | |
| chr1:167,066,432–167,066,980 | 99.7 kb | Distal (>10kb) Multiome | 430 | |
| chr1:167,148,291–167,148,891 | 17.9 kb | Distal (>10kb) Multiome | 99 | |
| chr1:167,165,942–167,166,725 | 23 bp | At TSS Multiome | 236 | |
| chr1:167,219,764–167,222,585 | 54.1 kb | Distal (>10kb) Multiome | 1143 | |
| chr1:167,345,001–167,345,700 | 178.9 kb | Distal (>10kb) Multiome | 142 |
Genomic view of the GPA33 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.