This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GLE1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GLE1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GLE1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:128,203,004–128,204,704 | 300.3 kb | Distal (>10kb) Multiome | 690 | |
| chr9:128,218,656–128,219,107 | 285.8 kb | Distal (>10kb) Multiome | 410 | |
| chr9:128,250,012–128,251,099 | 254.2 kb | Distal (>10kb) Multiome | 353 | |
| chr9:128,275,636–128,276,535 | 228.7 kb | Distal (>10kb) Multiome | 728 | |
| chr9:128,322,183–128,322,933 | 182.1 kb | Distal (>10kb) Multiome | 962 | |
| chr9:128,340,070–128,341,161 | 164.2 kb | Distal (>10kb) Multiome | 803 | |
| chr9:128,364,858–128,365,437 | 139.6 kb | Distal (>10kb) Multiome | 442 | |
| chr9:128,420,259–128,421,260 | 84.0 kb | Distal (>10kb) Multiome | 701 | |
| chr9:128,455,721–128,456,522 | 48.6 kb | Distal (>10kb) Multiome | 684 | |
| chr9:128,504,482–128,505,188 | 10 bp | At TSS Multiome | 766 | |
| chr9:128,552,006–128,553,125 | 47.8 kb | Distal (>10kb) Multiome | 839 | |
| chr9:128,656,436–128,657,126 | 152.1 kb | Distal (>10kb) Multiome | 1009 | |
| chr9:128,683,393–128,684,010 | 178.9 kb | Distal (>10kb) Multiome | 296 | |
| chr9:128,684,170–128,684,813 | 180.0 kb | Distal (>10kb) Multiome | 574 | |
| chr9:128,688,449–128,689,713 | 184.4 kb | Distal (>10kb) Multiome | 805 | |
| chr9:128,702,246–128,702,946 | 197.8 kb | Distal (>10kb) Multiome | 720 | |
| chr9:128,723,691–128,725,229 | 219.5 kb | Distal (>10kb) Multiome | 821 | |
| chr9:128,771,658–128,772,301 | 267.3 kb | Distal (>10kb) Multiome | 940 | |
| chr9:128,786,928–128,787,516 | 282.5 kb | Distal (>10kb) Multiome | 1040 |
Genomic view of the GLE1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.