Transcription factors with Perturb-seq knockdown data for GIRGL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GIRGL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GIRGL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:20,214,924–20,215,519 | 2.1 kb | Proximal (<10kb) | 161 | |
| chr7:20,217,098–20,217,914 | at TSS | At TSS | 460 | |
| chr7:20,218,398–20,218,569 | 822 bp | At TSS | 46 | |
| chr7:20,219,096–20,220,603 | 1.5 kb | Proximal (<10kb) | 899 | |
| chr7:20,226,067–20,226,279 | 8.5 kb | Proximal (<10kb) | 2 |
Genomic view of the GIRGL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.