Transcription factors with Perturb-seq knockdown data for GIHCG. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GIHCG upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GIHCG, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:57,693,486–57,694,647 | 242.2 kb | Distal (>10kb) Multiome | 757 | |
| chr12:57,726,079–57,726,879 | 209.8 kb | Distal (>10kb) Multiome | 597 | |
| chr12:57,744,434–57,745,735 | 191.1 kb | Distal (>10kb) Multiome | 822 | |
| chr12:57,751,806–57,753,147 | 183.6 kb | Distal (>10kb) Multiome | 1030 | |
| chr12:57,754,455–57,756,188 | 181.2 kb | Distal (>10kb) Multiome | 616 | |
| chr12:57,765,020–57,765,720 | 170.8 kb | Distal (>10kb) Multiome | 472 | |
| chr12:57,766,452–57,767,514 | 169.1 kb | Distal (>10kb) Multiome | 330 | |
| chr12:57,771,528–57,773,018 | 164.0 kb | Distal (>10kb) Multiome | 1006 | |
| chr12:57,782,579–57,783,204 | 153.4 kb | Distal (>10kb) Multiome | 916 | |
| chr12:57,844,422–57,847,797 | 89.1 kb | Distal (>10kb) Multiome | 1032 | |
| chr12:57,852,281–57,853,469 | 83.3 kb | Distal (>10kb) Multiome | 295 | |
| chr12:57,896,473–57,897,915 | 39.2 kb | Distal (>10kb) Multiome | 419 | |
| chr12:57,935,860–57,936,461 | 50 bp | At TSS Multiome | 650 | |
| chr12:57,940,778–57,942,227 | 5.4 kb | Proximal (<10kb) Multiome | 836 | |
| chr12:58,139,396–58,140,263 | 203.9 kb | Distal (>10kb) Multiome | 150 | |
| chr12:58,206,630–58,207,741 | 271.0 kb | Distal (>10kb) Multiome | 155 | |
| chr12:59,074,983–59,076,039 | 1139.4 kb | Distal (>10kb) Multiome HiCAR | 168 |
Genomic view of the GIHCG locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.