Transcription factors with Perturb-seq knockdown data for GASK1B-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GASK1B-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GASK1B-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:158,161,717–158,161,915 | 8.8 kb | Proximal (<10kb) | 169 | |
| chr4:158,170,430–158,171,299 | at TSS | At TSS | 438 | |
| chr4:158,172,368–158,172,491 | 1.6 kb | Proximal (<10kb) | 77 | |
| chr4:158,172,608–158,172,849 | 1.9 kb | Proximal (<10kb) | 243 | |
| chr4:158,176,168–158,176,644 | 5.4 kb | Proximal (<10kb) | 96 |
Genomic view of the GASK1B-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.