Transcription factors with Perturb-seq knockdown data for GASAL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GASAL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GASAL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:102,806,122–102,807,887 | at TSS | At TSS | 1159 | |
| chr8:102,808,604–102,808,775 | 931 bp | At TSS | 690 | |
| chr8:102,810,067–102,811,695 | 2.4 kb | Proximal (<10kb) | 1025 | |
| chr8:102,814,226–102,814,970 | 6.6 kb | Proximal (<10kb) | 366 |
Genomic view of the GASAL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.