Transcription factors with Perturb-seq knockdown data for GAPDH-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GAPDH-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GAPDH-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:6,533,253–6,535,726 | at TSS | At TSS | 1131 | |
| chr12:6,537,710–6,538,506 | 4.2 kb | Proximal (<10kb) | 310 | |
| chr12:6,538,996–6,539,244 | 5.5 kb | Proximal (<10kb) | 236 | |
| chr12:6,539,914–6,540,883 | 6.4 kb | Proximal (<10kb) | 427 |
Genomic view of the GAPDH-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.