The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]
Transcription factors with Perturb-seq knockdown data for FKBP4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FKBP4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FKBP4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:2,691,016–2,691,920 | 103.3 kb | Distal (>10kb) Multiome | 194 | |
| chr12:2,752,645–2,753,234 | 42.2 kb | Distal (>10kb) Multiome | 945 | |
| chr12:2,794,243–2,796,266 | 992 bp | At TSS Multiome | 802 | |
| chr12:2,812,386–2,813,317 | 17.6 kb | Distal (>10kb) Multiome HiCAR | 1037 | |
| chr12:2,845,941–2,846,561 | 51.1 kb | Distal (>10kb) Multiome | 220 | |
| chr12:2,876,673–2,877,899 | 82.2 kb | Distal (>10kb) Multiome | 809 | |
| chr12:2,890,285–2,891,599 | 95.7 kb | Distal (>10kb) Multiome | 880 | |
| chr12:2,958,171–2,960,222 | 164.3 kb | Distal (>10kb) Multiome | 770 | |
| chr12:3,076,742–3,077,982 | 282.2 kb | Distal (>10kb) Multiome | 614 |
Genomic view of the FKBP4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.