This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. It shares 78% sequence identity with the mouse protein. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for FBXL8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FBXL8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FBXL8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:67,149,282–67,151,406 | 8.5 kb | Proximal (<10kb) | 693 | |
| chr16:67,154,687–67,155,820 | 4.1 kb | Proximal (<10kb) | 593 | |
| chr16:67,159,109–67,160,527 | at TSS | At TSS | 721 | |
| chr16:67,160,628–67,161,206 | 697 bp | At TSS | 524 | |
| chr16:67,162,969–67,163,815 | 3.0 kb | Proximal (<10kb) | 238 | |
| chr16:67,169,105–67,171,287 | 9.2 kb | Proximal (<10kb) | 813 |
Genomic view of the FBXL8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.