Transcription factors with Perturb-seq knockdown data for FAM149A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FAM149A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FAM149A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:185,838,997–185,839,673 | 265.3 kb | Distal (>10kb) Multiome | 329 | |
| chr4:186,081,208–186,081,652 | 23.3 kb | Distal (>10kb) Multiome | 58 | |
| chr4:186,104,365–186,105,711 | 112 bp | At TSS Multiome | 349 | |
| chr4:186,144,033–186,145,441 | 40.2 kb | Distal (>10kb) Multiome | 389 | |
| chr4:186,149,907–186,150,302 | 5.4 kb | Proximal (<10kb) | 125 | |
| chr4:186,190,592–186,192,541 | 86.9 kb | Distal (>10kb) Multiome | 993 |
Genomic view of the FAM149A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.