ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for ERRFI1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ERRFI1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ERRFI1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:7,770,840–7,771,872 | 255.0 kb | Distal (>10kb) Multiome | 711 | |
| chr1:7,783,377–7,785,071 | 242.4 kb | Distal (>10kb) Multiome | 618 | |
| chr1:7,885,362–7,886,254 | 140.6 kb | Distal (>10kb) Multiome | 196 | |
| chr1:7,942,207–7,942,705 | 83.8 kb | Distal (>10kb) Multiome | 315 | |
| chr1:7,953,925–7,954,593 | 72.1 kb | Distal (>10kb) Multiome | 708 | |
| chr1:7,961,111–7,962,145 | 64.7 kb | Distal (>10kb) Multiome | 1012 | |
| chr1:8,004,118–8,005,226 | 21.6 kb | Distal (>10kb) Multiome | 617 | |
| chr1:8,022,716–8,023,612 | 2.7 kb | Proximal (<10kb) | 100 | |
| chr1:8,025,593–8,027,129 | 70 bp | At TSS Multiome | 938 | |
| chr1:8,027,270–8,027,546 | 961 bp | At TSS | 469 | |
| chr1:8,083,702–8,084,519 | 57.9 kb | Distal (>10kb) Multiome | 382 | |
| chr1:8,107,730–8,108,244 | 81.8 kb | Distal (>10kb) Multiome | 104 | |
| chr1:8,166,706–8,167,855 | 141.1 kb | Distal (>10kb) Multiome HiCAR | 348 | |
| chr1:8,216,976–8,217,707 | 190.9 kb | Distal (>10kb) Multiome HiCAR | 224 |
Genomic view of the ERRFI1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.