Transcription factors with Perturb-seq knockdown data for EOGT-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EOGT-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EOGT-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:68,931,297–68,933,078 | 81.3 kb | Distal (>10kb) Multiome | 419 | |
| chr3:69,008,935–69,010,149 | 3.8 kb | Proximal (<10kb) | 309 | |
| chr3:69,012,112–69,014,102 | 314 bp | At TSS Multiome | 935 | |
| chr3:69,079,501–69,080,755 | 66.4 kb | Distal (>10kb) Multiome | 569 | |
| chr3:69,084,409–69,085,904 | 71.0 kb | Distal (>10kb) Multiome | 707 | |
| chr3:69,288,126–69,288,883 | 274.5 kb | Distal (>10kb) Multiome | 254 | |
| chr3:69,290,108–69,290,999 | 276.7 kb | Distal (>10kb) Multiome | 171 |
Genomic view of the EOGT-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.