Transcription factors with Perturb-seq knockdown data for EN2-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EN2-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EN2-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:155,448,617–155,449,076 | 8.0 kb | Proximal (<10kb) | 118 | |
| chr7:155,449,181–155,450,383 | 6.7 kb | Proximal (<10kb) | 142 | |
| chr7:155,453,574–155,455,097 | 2.0 kb | Proximal (<10kb) | 119 | |
| chr7:155,456,809–155,459,720 | at TSS | At TSS | 434 | |
| chr7:155,466,156–155,467,479 | 9.1 kb | Proximal (<10kb) | 270 |
Genomic view of the EN2-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.