EME2

essential meiotic structure-specific endonuclease subunit 2 | FLJ00151, SLX2B

EME2 forms a heterodimer with MUS81 (MIM 606591) that functions as an XPF (MIM 278760)-type flap/fork endonuclease in DNA repair (Ciccia et al., 2007 [PubMed 17289582]).[supplied by OMIM, Mar 2008]

Developmental clusters: GC7

Biological processes 26 terms

DNA binding (GO:0003677)DNA endonuclease activity (GO:0004520)DNA endonuclease activity (GO:0004520)DNA repair (GO:0006281)Holliday junction resolvase complex (GO:0048476)Holliday junction resolvase complex (GO:0048476)chromosome, telomeric region (GO:0000781)double-strand break repair (GO:0006302)double-strand break repair (GO:0006302)double-strand break repair (GO:0006302)endodeoxyribonuclease complex (GO:1905347)endodeoxyribonuclease complex (GO:1905347)enzyme-substrate adaptor activity (GO:0140767)mitotic intra-S DNA damage checkpoint signaling (GO:0031573)nuclear chromosome (GO:0000228)nuclear replication fork (GO:0043596)nuclear replication fork (GO:0043596)nucleus (GO:0005634)protein binding (GO:0005515)replication fork processing (GO:0031297)replication fork processing (GO:0031297)replication fork processing (GO:0031297)replication fork processing (GO:0031297)resolution of meiotic recombination intermediates (GO:0000712)resolution of mitotic recombination intermediates (GO:0071140)telomere maintenance (GO:0000723)

Expression (TPM)

TFs regulating EME2 0 TFs

Transcription factors with Perturb-seq knockdown data for EME2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EME2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to EME2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EME2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr16:1,474,566–1,475,411	298.2 kb	Distal (>10kb) Multiome	915
chr16:1,492,797–1,493,962	280.0 kb	Distal (>10kb) Multiome HiCAR	710
chr16:1,610,035–1,611,264	162.7 kb	Distal (>10kb) Multiome	745
chr16:1,611,870–1,612,834	161.1 kb	Distal (>10kb) Multiome	718
chr16:1,677,899–1,679,024	95.1 kb	Distal (>10kb) Multiome	709
chr16:1,705,380–1,706,702	67.2 kb	Distal (>10kb) Multiome	933
chr16:1,763,511–1,764,353	9.3 kb	Proximal (<10kb) Multiome	261
chr16:1,770,847–1,771,973	1.7 kb	Proximal (<10kb) Multiome	485
chr16:1,772,565–1,774,049	67 bp	At TSS Multiome	956
chr16:1,781,749–1,783,420	9.4 kb	Proximal (<10kb) Multiome	761
chr16:1,826,102–1,827,535	53.6 kb	Distal (>10kb) Multiome	1006
chr16:1,918,171–1,918,773	145.1 kb	Distal (>10kb) Multiome	129
chr16:1,929,283–1,930,271	156.5 kb	Distal (>10kb) Multiome	780
chr16:1,942,671–1,943,761	170.0 kb	Distal (>10kb) Multiome	750
chr16:1,958,923–1,960,140	186.2 kb	Distal (>10kb) Multiome	826
chr16:1,964,300–1,965,434	191.6 kb	Distal (>10kb) Multiome	933
chr16:1,971,481–1,972,435	198.6 kb	Distal (>10kb) Multiome	1010
chr16:1,979,111–1,980,324	206.1 kb	Distal (>10kb) Multiome	296
chr16:1,983,450–1,984,851	210.6 kb	Distal (>10kb) Multiome	917
chr16:1,989,307–1,993,568	219.1 kb	Distal (>10kb) Multiome	706
chr16:2,003,882–2,004,348	230.7 kb	Distal (>10kb) Multiome	619
chr16:2,009,742–2,010,504	236.5 kb	Distal (>10kb) Multiome	752
chr16:2,022,700–2,023,923	249.9 kb	Distal (>10kb) Multiome	682
chr16:2,026,629–2,027,149	253.5 kb	Distal (>10kb) Multiome	249
chr16:2,047,366–2,048,252	274.5 kb	Distal (>10kb) Multiome	875

Genome Browser

Genomic view of the EME2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.