Transcription factors with Perturb-seq knockdown data for EHMT2-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EHMT2-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EHMT2-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:31,619,744–31,621,435 | 263.2 kb | Distal (>10kb) Multiome | 1127 | |
| chr6:31,651,682–31,653,297 | 231.1 kb | Distal (>10kb) Multiome | 1113 | |
| chr6:31,660,325–31,660,941 | 222.9 kb | Distal (>10kb) Multiome | 892 | |
| chr6:31,664,725–31,666,373 | 218.9 kb | Distal (>10kb) Multiome | 1039 | |
| chr6:31,682,887–31,683,880 | 200.4 kb | Distal (>10kb) Multiome | 248 | |
| chr6:31,702,816–31,703,520 | 180.5 kb | Distal (>10kb) Multiome | 875 | |
| chr6:31,728,852–31,730,814 | 154.1 kb | Distal (>10kb) Multiome | 979 | |
| chr6:31,739,236–31,740,741 | 143.8 kb | Distal (>10kb) Multiome | 1043 | |
| chr6:31,766,045–31,766,778 | 117.3 kb | Distal (>10kb) Multiome | 351 | |
| chr6:31,795,097–31,796,366 | 87.8 kb | Distal (>10kb) Multiome | 979 | |
| chr6:31,806,162–31,807,183 | 76.8 kb | Distal (>10kb) Multiome | 947 | |
| chr6:31,814,125–31,815,936 | 69.1 kb | Distal (>10kb) Multiome | 822 | |
| chr6:31,821,306–31,822,544 | 61.8 kb | Distal (>10kb) Multiome | 714 | |
| chr6:31,826,770–31,828,135 | 56.3 kb | Distal (>10kb) Multiome | 1000 | |
| chr6:31,834,277–31,835,371 | 49.0 kb | Distal (>10kb) Multiome | 1085 | |
| chr6:31,862,148–31,864,173 | 19.8 kb | Distal (>10kb) Multiome | 1118 | |
| chr6:31,896,965–31,898,037 | 13.8 kb | Distal (>10kb) Multiome | 654 | |
| chr6:31,901,114–31,902,643 | 18.5 kb | Distal (>10kb) Multiome | 826 | |
| chr6:31,958,527–31,959,598 | 75.3 kb | Distal (>10kb) Multiome | 953 | |
| chr6:31,971,421–31,972,872 | 88.5 kb | Distal (>10kb) Multiome | 1059 | |
| chr6:32,048,217–32,048,679 | 164.6 kb | Distal (>10kb) Multiome | 611 | |
| chr6:32,087,265–32,088,160 | 204.0 kb | Distal (>10kb) Multiome | 473 | |
| chr6:32,127,621–32,128,720 | 244.5 kb | Distal (>10kb) Multiome | 683 | |
| chr6:32,129,973–32,130,800 | 246.6 kb | Distal (>10kb) Multiome | 717 | |
| chr6:32,148,431–32,149,344 | 265.0 kb | Distal (>10kb) Multiome | 145 | |
| chr6:32,153,307–32,155,059 | 270.2 kb | Distal (>10kb) Multiome | 908 | |
| chr6:32,175,673–32,176,347 | 292.4 kb | Distal (>10kb) Multiome | 654 | |
| chr6:32,177,078–32,178,952 | 294.3 kb | Distal (>10kb) Multiome | 820 |
Genomic view of the EHMT2-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.