This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
Transcription factors with Perturb-seq knockdown data for EGF. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EGF upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EGF, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:109,701,412–109,703,899 | 209.5 kb | Distal (>10kb) Multiome | 799 | |
| chr4:109,729,247–109,730,761 | 182.8 kb | Distal (>10kb) Multiome | 793 | |
| chr4:109,814,867–109,816,081 | 97.4 kb | Distal (>10kb) Multiome | 846 | |
| chr4:109,846,257–109,847,050 | 66.2 kb | Distal (>10kb) Multiome HiCAR | 131 | |
| chr4:109,912,685–109,913,476 | at TSS | At TSS | 180 | |
| chr4:109,928,231–109,928,964 | 15.7 kb | Distal (>10kb) Multiome | 114 | |
| chr4:110,132,887–110,133,666 | 220.4 kb | Distal (>10kb) Multiome | 212 | |
| chr4:110,195,911–110,198,252 | 283.7 kb | Distal (>10kb) Multiome | 806 | |
| chr4:110,198,361–110,199,448 | 285.9 kb | Distal (>10kb) Multiome | 817 |
Genomic view of the EGF locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.