This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
Transcription factors with Perturb-seq knockdown data for ECI1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ECI1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ECI1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:1,958,923–1,960,140 | 292.1 kb | Distal (>10kb) Multiome | 826 | |
| chr16:1,964,300–1,965,434 | 286.7 kb | Distal (>10kb) Multiome | 933 | |
| chr16:1,971,481–1,972,435 | 279.6 kb | Distal (>10kb) Multiome | 1010 | |
| chr16:1,979,111–1,980,324 | 272.2 kb | Distal (>10kb) Multiome | 296 | |
| chr16:1,983,450–1,984,851 | 267.6 kb | Distal (>10kb) Multiome | 917 | |
| chr16:1,989,307–1,993,568 | 259.2 kb | Distal (>10kb) Multiome | 706 | |
| chr16:2,003,882–2,004,348 | 247.5 kb | Distal (>10kb) Multiome | 619 | |
| chr16:2,009,742–2,010,504 | 241.7 kb | Distal (>10kb) Multiome | 752 | |
| chr16:2,022,700–2,023,923 | 228.3 kb | Distal (>10kb) Multiome | 682 | |
| chr16:2,026,629–2,027,149 | 224.7 kb | Distal (>10kb) Multiome | 249 | |
| chr16:2,047,366–2,048,252 | 203.7 kb | Distal (>10kb) Multiome | 875 | |
| chr16:2,090,476–2,092,232 | 160.3 kb | Distal (>10kb) Multiome | 344 | |
| chr16:2,135,536–2,136,505 | 115.5 kb | Distal (>10kb) Multiome | 263 | |
| chr16:2,147,864–2,149,260 | 103.1 kb | Distal (>10kb) Multiome | 679 | |
| chr16:2,149,550–2,152,180 | 100.5 kb | Distal (>10kb) Multiome | 618 | |
| chr16:2,152,861–2,153,999 | 97.9 kb | Distal (>10kb) Multiome | 734 | |
| chr16:2,154,834–2,156,329 | 96.0 kb | Distal (>10kb) Multiome | 932 | |
| chr16:2,178,140–2,179,722 | 72.8 kb | Distal (>10kb) Multiome | 318 | |
| chr16:2,205,129–2,206,297 | 46.1 kb | Distal (>10kb) Multiome | 887 | |
| chr16:2,214,212–2,215,803 | 36.6 kb | Distal (>10kb) Multiome | 953 | |
| chr16:2,223,095–2,224,285 | 28.1 kb | Distal (>10kb) Multiome | 918 | |
| chr16:2,251,044–2,252,460 | 87 bp | At TSS Multiome | 996 | |
| chr16:2,267,625–2,269,111 | 16.7 kb | Distal (>10kb) Multiome | 1044 | |
| chr16:2,339,631–2,342,283 | 89.3 kb | Distal (>10kb) Multiome | 1129 | |
| chr16:2,428,282–2,429,948 | 177.7 kb | Distal (>10kb) Multiome | 827 | |
| chr16:2,459,524–2,460,612 | 208.5 kb | Distal (>10kb) Multiome | 670 | |
| chr16:2,467,565–2,468,324 | 216.2 kb | Distal (>10kb) Multiome | 450 | |
| chr16:2,470,913–2,472,223 | 219.7 kb | Distal (>10kb) Multiome | 427 | |
| chr16:2,473,455–2,476,127 | 223.6 kb | Distal (>10kb) Multiome | 719 | |
| chr16:2,501,124–2,501,877 | 249.9 kb | Distal (>10kb) Multiome | 471 | |
| chr16:2,513,006–2,514,809 | 262.2 kb | Distal (>10kb) Multiome | 818 | |
| chr16:2,520,064–2,520,618 | 268.8 kb | Distal (>10kb) Multiome | 624 | |
| chr16:2,531,456–2,533,080 | 280.5 kb | Distal (>10kb) Multiome | 883 | |
| chr16:2,537,433–2,538,433 | 286.3 kb | Distal (>10kb) Multiome | 498 |
Genomic view of the ECI1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.