This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for EBAG9. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EBAG9 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EBAG9, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:109,262,011–109,263,133 | 277.3 kb | Distal (>10kb) Multiome | 324 | |
| chr8:109,281,877–109,282,698 | 257.5 kb | Distal (>10kb) Multiome | 264 | |
| chr8:109,333,269–109,335,026 | 205.4 kb | Distal (>10kb) Multiome | 1002 | |
| chr8:109,361,479–109,363,684 | 177.7 kb | Distal (>10kb) Multiome HiCAR | 529 | |
| chr8:109,537,263–109,537,684 | 2.0 kb | Proximal (<10kb) | 87 | |
| chr8:109,538,966–109,540,699 | 89 bp | At TSS Multiome | 1183 | |
| chr8:109,643,972–109,645,811 | 105.1 kb | Distal (>10kb) Multiome | 815 | |
| chr8:109,690,762–109,692,230 | 151.9 kb | Distal (>10kb) Multiome | 449 |
Genomic view of the EBAG9 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.