E4F1

E4F transcription factor 1 | E4F

The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. Alternative splicing results in multiple transcripts encoding different proteins. [provided by RefSeq, Jan 2014]

Biological processes 24 terms

DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)RNA polymerase II-specific DNA-binding transcription factor binding (GO:0061629)cAMP response element binding (GO:0035497)cytoplasm (GO:0005737)negative regulation of transcription by RNA polymerase II (GO:0000122)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)protein ubiquitination (GO:0016567)regulation of cell cycle (GO:0051726)regulation of cell cycle process (GO:0010564)regulation of cell cycle process (GO:0010564)regulation of transcription by RNA polymerase II (GO:0006357)spindle (GO:0005819)ubiquitin protein ligase activity (GO:0061630)

Expression (TPM)

TFs regulating E4F1 0 TFs

Transcription factors with Perturb-seq knockdown data for E4F1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = E4F1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to E4F1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of E4F1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr16:1,929,283–1,930,271	293.8 kb	Distal (>10kb) Multiome	780
chr16:1,942,671–1,943,761	280.2 kb	Distal (>10kb) Multiome	750
chr16:1,958,923–1,960,140	264.1 kb	Distal (>10kb) Multiome	826
chr16:1,964,300–1,965,434	258.7 kb	Distal (>10kb) Multiome	933
chr16:1,971,481–1,972,435	251.6 kb	Distal (>10kb) Multiome	1010
chr16:1,979,111–1,980,324	244.2 kb	Distal (>10kb) Multiome	296
chr16:1,983,450–1,984,851	239.7 kb	Distal (>10kb) Multiome	917
chr16:1,989,307–1,993,568	231.2 kb	Distal (>10kb) Multiome	706
chr16:2,003,882–2,004,348	219.6 kb	Distal (>10kb) Multiome	619
chr16:2,009,742–2,010,504	213.7 kb	Distal (>10kb) Multiome	752
chr16:2,022,700–2,023,923	200.3 kb	Distal (>10kb) Multiome	682
chr16:2,026,629–2,027,149	196.7 kb	Distal (>10kb) Multiome	249
chr16:2,047,366–2,048,252	175.7 kb	Distal (>10kb) Multiome	875
chr16:2,090,476–2,092,232	132.3 kb	Distal (>10kb) Multiome	344
chr16:2,135,536–2,136,505	87.5 kb	Distal (>10kb) Multiome	263
chr16:2,147,864–2,149,260	75.1 kb	Distal (>10kb) Multiome	679
chr16:2,149,550–2,152,180	72.5 kb	Distal (>10kb) Multiome	618
chr16:2,152,861–2,153,999	69.9 kb	Distal (>10kb) Multiome	734
chr16:2,154,834–2,156,329	68.1 kb	Distal (>10kb) Multiome	932
chr16:2,178,140–2,179,722	44.8 kb	Distal (>10kb) Multiome	318
chr16:2,205,129–2,206,297	18.1 kb	Distal (>10kb) Multiome	887
chr16:2,214,212–2,215,803	8.6 kb	Proximal (<10kb) Multiome	953
chr16:2,223,095–2,224,285	136 bp	At TSS Multiome	918
chr16:2,251,044–2,252,460	28.1 kb	Distal (>10kb) Multiome	996
chr16:2,267,625–2,269,111	44.6 kb	Distal (>10kb) Multiome	1044
chr16:2,339,631–2,342,283	117.2 kb	Distal (>10kb) Multiome	1129
chr16:2,428,282–2,429,948	205.7 kb	Distal (>10kb) Multiome	827
chr16:2,459,524–2,460,612	236.5 kb	Distal (>10kb) Multiome	670
chr16:2,467,565–2,468,324	244.2 kb	Distal (>10kb) Multiome	450
chr16:2,470,913–2,472,223	247.7 kb	Distal (>10kb) Multiome	427
chr16:2,473,455–2,476,127	251.6 kb	Distal (>10kb) Multiome	719
chr16:2,501,124–2,501,877	277.8 kb	Distal (>10kb) Multiome	471
chr16:2,513,006–2,514,809	290.2 kb	Distal (>10kb) Multiome	818
chr16:2,520,064–2,520,618	296.7 kb	Distal (>10kb) Multiome	624

Genome Browser

Genomic view of the E4F1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.