E2F2

E2F transcription factor 2 | E2F-2

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008]

Developmental clusters: GC7

Biological processes 27 terms

DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II transcription regulator complex (GO:0090575)RNA polymerase II transcription regulator complex (GO:0090575)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)chromatin (GO:0000785)cis-regulatory region sequence-specific DNA binding (GO:0000987)negative regulation of sprouting angiogenesis (GO:1903671)nucleoplasm (GO:0005654)nucleus (GO:0005634)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)protein dimerization activity (GO:0046983)regulation of DNA-templated transcription (GO:0006355)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)sequence-specific DNA binding (GO:0043565)sequence-specific double-stranded DNA binding (GO:1990837)transcription initiation at RNA polymerase II promoter (GO:0006367)transcription regulator complex (GO:0005667)

Expression (TPM)

TFs regulating E2F2 0 TFs

Transcription factors with Perturb-seq knockdown data for E2F2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = E2F2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to E2F2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of E2F2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr1:23,343,731–23,344,999	186.8 kb	Distal (>10kb) Multiome	939
chr1:23,367,863–23,369,864	162.7 kb	Distal (>10kb) Multiome	1018
chr1:23,370,752–23,371,596	160.1 kb	Distal (>10kb) Multiome	344
chr1:23,436,460–23,437,402	94.4 kb	Distal (>10kb) Multiome	248
chr1:23,483,306–23,485,071	46.8 kb	Distal (>10kb) Multiome	659
chr1:23,530,568–23,531,711	69 bp	At TSS Multiome	775
chr1:23,539,704–23,540,280	8.8 kb	Proximal (<10kb) Multiome	668
chr1:23,554,367–23,556,627	23.7 kb	Distal (>10kb) Multiome	961
chr1:23,558,135–23,559,925	28.3 kb	Distal (>10kb) Multiome	932
chr1:23,598,114–23,599,073	67.4 kb	Distal (>10kb) Multiome	329
chr1:23,691,587–23,692,258	160.5 kb	Distal (>10kb) Multiome	876
chr1:23,742,958–23,744,137	212.1 kb	Distal (>10kb) Multiome	802
chr1:23,777,908–23,778,814	247.2 kb	Distal (>10kb) Multiome	849
chr1:23,790,777–23,791,448	259.9 kb	Distal (>10kb) Multiome	637
chr1:23,799,493–23,801,538	269.6 kb	Distal (>10kb) Multiome	922
chr1:23,825,075–23,825,633	294.2 kb	Distal (>10kb) Multiome	867

Genome Browser

Genomic view of the E2F2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.