Transcription factors with Perturb-seq knockdown data for DSP-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DSP-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DSP-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:7,260,713–7,262,501 | 280.0 kb | Distal (>10kb) Multiome | 852 | |
| chr6:7,312,560–7,313,754 | 228.2 kb | Distal (>10kb) Multiome | 1075 | |
| chr6:7,347,359–7,348,352 | 193.7 kb | Distal (>10kb) Multiome | 256 | |
| chr6:7,389,283–7,390,343 | 151.6 kb | Distal (>10kb) Multiome | 1040 | |
| chr6:7,532,325–7,532,503 | 8.9 kb | Proximal (<10kb) | 140 | |
| chr6:7,540,580–7,540,874 | 543 bp | At TSS | 138 | |
| chr6:7,541,127–7,543,319 | 139 bp | At TSS Multiome | 970 | |
| chr6:7,548,319–7,549,268 | 6.9 kb | Proximal (<10kb) | 504 | |
| chr6:7,589,842–7,590,956 | 48.8 kb | Distal (>10kb) Multiome | 877 | |
| chr6:7,672,770–7,673,478 | 131.8 kb | Distal (>10kb) Multiome | 337 | |
| chr6:7,725,533–7,728,906 | 184.6 kb | Distal (>10kb) Multiome | 639 |
Genomic view of the DSP-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.