Transcription factors with Perturb-seq knockdown data for DSEL-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DSEL-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DSEL-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:67,368,248–67,369,001 | 147.9 kb | Distal (>10kb) Multiome | 96 | |
| chr18:67,455,460–67,456,258 | 60.6 kb | Distal (>10kb) Multiome | 86 | |
| chr18:67,515,663–67,517,723 | 619 bp | At TSS Multiome | 579 | |
| chr18:67,525,347–67,525,694 | 8.8 kb | Proximal (<10kb) | 116 | |
| chr18:67,585,750–67,587,104 | 69.8 kb | Distal (>10kb) Multiome | 152 | |
| chr18:67,590,473–67,591,397 | 74.3 kb | Distal (>10kb) Multiome | 155 | |
| chr18:68,561,806–68,563,075 | 1045.8 kb | Distal (>10kb) Multiome HiCAR | 132 | |
| chr18:68,714,344–68,715,550 | 1198.6 kb | Distal (>10kb) Multiome HiCAR | 900 |
Genomic view of the DSEL-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.