The mouse and human DIO3OS and DIO3 (MIM 601038) genes overlap and are transcribed in opposite directions. The mouse Dio3 gene is imprinted from the paternal allele during fetal development, suggesting that DIO3OS is a noncoding gene that may have a role in maintaining monoallelic expression of DIO3 (Hernandez et al., 2004 [PubMed 14962667]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for DIO3OS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DIO3OS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DIO3OS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:100,904,412–100,905,040 | 655.7 kb | Distal (>10kb) Multiome HiCAR | 121 | |
| chr14:101,183,321–101,183,789 | 376.9 kb | Distal (>10kb) Multiome HiCAR | 167 | |
| chr14:101,310,915–101,311,513 | 249.2 kb | Distal (>10kb) Multiome | 8 | |
| chr14:101,458,642–101,459,586 | 101.1 kb | Distal (>10kb) Multiome | 189 | |
| chr14:101,559,493–101,562,183 | 1.0 kb | Proximal (<10kb) Multiome | 329 | |
| chr14:101,563,518–101,564,657 | 3.7 kb | Proximal (<10kb) Multiome | 127 | |
| chr14:101,564,868–101,565,264 | 4.7 kb | Proximal (<10kb) | 120 | |
| chr14:101,688,293–101,688,782 | 128.2 kb | Distal (>10kb) Multiome | 137 | |
| chr14:101,705,720–101,706,457 | 145.8 kb | Distal (>10kb) Multiome | 326 | |
| chr14:101,726,532–101,727,181 | 166.4 kb | Distal (>10kb) Multiome | 45 | |
| chr14:101,760,098–101,762,537 | 201.4 kb | Distal (>10kb) Multiome | 852 | |
| chr14:101,809,684–101,810,434 | 249.7 kb | Distal (>10kb) Multiome | 701 |
Genomic view of the DIO3OS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.