Enables dihydrofolate reductase activity and mRNA binding activity. Involved in tetrahydrofolate metabolic process and thymidine biosynthetic process. Located in mitochondrial inner membrane and mitochondrial matrix. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for DHFR2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DHFR2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DHFR2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:93,973,221–93,974,313 | 89.1 kb | Distal (>10kb) Multiome | 755 | |
| chr3:93,979,611–93,980,934 | 82.8 kb | Distal (>10kb) Multiome | 702 | |
| chr3:94,062,348–94,063,486 | 57 bp | At TSS Multiome | 797 | |
| chr3:94,066,379–94,067,550 | 4.0 kb | Proximal (<10kb) Multiome | 160 | |
| chr3:94,250,605–94,251,787 | 188.3 kb | Distal (>10kb) Multiome | 210 | |
| chr3:94,256,923–94,257,930 | 194.5 kb | Distal (>10kb) Multiome | 219 |
Genomic view of the DHFR2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.