Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]
Transcription factors with Perturb-seq knockdown data for DGKB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DGKB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DGKB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:14,902,329–14,903,936 | at TSS | At TSS | 273 | |
| chr7:15,015,258–15,016,398 | 113.0 kb | Distal (>10kb) Multiome | 428 | |
| chr7:15,053,852–15,055,000 | 151.7 kb | Distal (>10kb) Multiome | 86 | |
| chr7:15,126,400–15,127,271 | 224.1 kb | Distal (>10kb) Multiome | 92 | |
| chr7:16,420,192–16,422,117 | 1518.2 kb | Distal (>10kb) Multiome HiCAR | 1014 |
Genomic view of the DGKB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.