DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for DEF6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DEF6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DEF6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:35,141,228–35,141,736 | 156.4 kb | Distal (>10kb) Multiome | 171 | |
| chr6:35,213,536–35,214,675 | 83.8 kb | Distal (>10kb) Multiome | 310 | |
| chr6:35,258,904–35,260,476 | 38.1 kb | Distal (>10kb) Multiome | 1142 | |
| chr6:35,294,424–35,294,613 | 3.2 kb | Proximal (<10kb) | 169 | |
| chr6:35,297,619–35,298,109 | 12 bp | At TSS Multiome | 765 | |
| chr6:35,317,884–35,318,871 | 20.7 kb | Distal (>10kb) Multiome | 727 | |
| chr6:35,342,126–35,343,068 | 44.7 kb | Distal (>10kb) Multiome | 831 | |
| chr6:35,451,927–35,453,018 | 154.5 kb | Distal (>10kb) Multiome HiCAR | 799 | |
| chr6:35,467,935–35,469,331 | 170.5 kb | Distal (>10kb) Multiome HiCAR | 1091 | |
| chr6:35,485,138–35,486,890 | 188.7 kb | Distal (>10kb) Multiome | 400 | |
| chr6:35,490,011–35,490,813 | 192.5 kb | Distal (>10kb) Multiome | 310 | |
| chr6:35,495,972–35,498,486 | 199.3 kb | Distal (>10kb) Multiome | 939 | |
| chr6:35,522,668–35,523,295 | 225.1 kb | Distal (>10kb) Multiome | 419 |
Genomic view of the DEF6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.