This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DDOST. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DDOST upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DDOST, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:20,366,975–20,367,437 | 294.1 kb | Distal (>10kb) Multiome | 164 | |
| chr1:20,402,741–20,403,363 | 258.4 kb | Distal (>10kb) Multiome | 165 | |
| chr1:20,430,474–20,430,904 | 230.7 kb | Distal (>10kb) Multiome | 217 | |
| chr1:20,485,233–20,485,959 | 175.7 kb | Distal (>10kb) Multiome | 348 | |
| chr1:20,486,131–20,487,438 | 174.6 kb | Distal (>10kb) Multiome | 760 | |
| chr1:20,493,822–20,494,241 | 167.3 kb | Distal (>10kb) Multiome | 430 | |
| chr1:20,507,717–20,508,432 | 153.2 kb | Distal (>10kb) Multiome | 811 | |
| chr1:20,552,019–20,553,890 | 109.0 kb | Distal (>10kb) Multiome | 518 | |
| chr1:20,588,675–20,590,358 | 72.4 kb | Distal (>10kb) Multiome | 431 | |
| chr1:20,625,186–20,625,881 | 35.9 kb | Distal (>10kb) Multiome | 123 | |
| chr1:20,633,024–20,634,989 | 27.5 kb | Distal (>10kb) Multiome | 759 | |
| chr1:20,661,099–20,662,130 | 167 bp | At TSS Multiome | 784 | |
| chr1:20,696,319–20,697,384 | 35.5 kb | Distal (>10kb) Multiome | 727 | |
| chr1:20,717,384–20,718,370 | 56.5 kb | Distal (>10kb) Multiome | 440 | |
| chr1:20,731,726–20,733,330 | 70.8 kb | Distal (>10kb) Multiome | 718 | |
| chr1:20,786,021–20,787,857 | 125.6 kb | Distal (>10kb) Multiome | 963 | |
| chr1:21,175,743–21,177,594 | 515.6 kb | Distal (>10kb) Multiome HiCAR | 807 |
Genomic view of the DDOST locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.