This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CYP11A1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CYP11A1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CYP11A1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:74,124,047–74,124,500 | 241.8 kb | Distal (>10kb) Multiome | 16 | |
| chr15:74,127,396–74,128,614 | 238.4 kb | Distal (>10kb) Multiome | 498 | |
| chr15:74,129,520–74,130,600 | 236.1 kb | Distal (>10kb) Multiome | 188 | |
| chr15:74,134,110–74,134,917 | 231.6 kb | Distal (>10kb) Multiome | 220 | |
| chr15:74,135,992–74,136,743 | 229.8 kb | Distal (>10kb) Multiome | 439 | |
| chr15:74,202,453–74,203,387 | 163.2 kb | Distal (>10kb) Multiome | 381 | |
| chr15:74,245,168–74,245,975 | 120.6 kb | Distal (>10kb) Multiome | 278 | |
| chr15:74,291,174–74,291,950 | 74.7 kb | Distal (>10kb) Multiome | 773 | |
| chr15:74,354,072–74,354,610 | 11.8 kb | Distal (>10kb) Multiome | 15 | |
| chr15:74,359,399–74,359,840 | 6.3 kb | Proximal (<10kb) | 18 | |
| chr15:74,365,599–74,366,233 | 211 bp | At TSS Multiome | 441 | |
| chr15:74,373,469–74,375,034 | 8.3 kb | Proximal (<10kb) Multiome | 745 | |
| chr15:74,375,313–74,375,510 | 9.2 kb | Proximal (<10kb) | 197 | |
| chr15:74,433,053–74,434,500 | 67.8 kb | Distal (>10kb) Multiome | 695 | |
| chr15:74,460,798–74,461,761 | 95.1 kb | Distal (>10kb) Multiome | 925 | |
| chr15:74,540,389–74,542,119 | 175.0 kb | Distal (>10kb) Multiome | 838 | |
| chr15:74,598,102–74,598,966 | 232.3 kb | Distal (>10kb) Multiome | 689 | |
| chr15:74,614,343–74,616,348 | 249.6 kb | Distal (>10kb) Multiome HiCAR | 1019 |
Genomic view of the CYP11A1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.