Enables heme binding activity. Contributes to nitrite reductase (NO-forming) activity. Involved in nitric oxide biosynthetic process. Located in membrane. Part of nitric-oxide synthase complex. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for CYB5B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CYB5B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CYB5B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:69,131,353–69,133,059 | 292.2 kb | Distal (>10kb) Multiome | 961 | |
| chr16:69,170,078–69,170,680 | 254.3 kb | Distal (>10kb) Multiome | 43 | |
| chr16:69,186,569–69,187,845 | 237.5 kb | Distal (>10kb) Multiome | 878 | |
| chr16:69,310,637–69,312,153 | 113.3 kb | Distal (>10kb) Multiome | 963 | |
| chr16:69,329,919–69,331,310 | 93.9 kb | Distal (>10kb) Multiome | 977 | |
| chr16:69,339,182–69,340,174 | 85.0 kb | Distal (>10kb) Multiome | 1014 | |
| chr16:69,385,555–69,386,170 | 38.7 kb | Distal (>10kb) Multiome | 857 | |
| chr16:69,424,128–69,425,019 | 95 bp | At TSS Multiome | 907 | |
| chr16:69,529,449–69,529,941 | 105.1 kb | Distal (>10kb) Multiome | 450 | |
| chr16:69,565,464–69,566,984 | 141.2 kb | Distal (>10kb) Multiome | 1044 |
Genomic view of the CYB5B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.