CX3CL1
C-X3-C motif chemokine ligand 1 | ABCD-3, C3Xkine, CXC3, CXC3C, NTN, SCYD1

This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017]

Biological processes 98 terms
CCR chemokine receptor binding (GO:0048020)CX3C chemokine receptor binding (GO:0031737)CX3C chemokine receptor binding (GO:0031737)CX3C chemokine receptor binding (GO:0031737)CX3C chemokine receptor binding (GO:0031737)CXCR1 chemokine receptor binding (GO:0045237)G protein-coupled receptor signaling pathway (GO:0007186)antimicrobial humoral immune response mediated by antimicrobial peptide (GO:0061844)autocrine signaling (GO:0035425)cell adhesion (GO:0007155)cell body (GO:0044297)cell chemotaxis (GO:0060326)cell projection (GO:0042995)cell surface (GO:0009986)cell surface (GO:0009986)cell-cell adhesion (GO:0098609)cell-cell adhesion (GO:0098609)cell-cell signaling (GO:0007267)chemoattractant activity (GO:0042056)chemokine activity (GO:0008009)chemokine activity (GO:0008009)chemokine activity (GO:0008009)chemokine activity (GO:0008009)chemokine activity (GO:0008009)chemokine-mediated signaling pathway (GO:0070098)chemokine-mediated signaling pathway (GO:0070098)chemokine-mediated signaling pathway (GO:0070098)chemotaxis (GO:0006935)chemotaxis (GO:0006935)cytokine-mediated signaling pathway (GO:0019221)defense response (GO:0006952)eosinophil chemotaxis (GO:0048245)extracellular region (GO:0005576)extracellular region (GO:0005576)extracellular region (GO:0005576)extracellular region (GO:0005576)extracellular region (GO:0005576)extracellular region (GO:0005576)immune response (GO:0006955)immune response (GO:0006955)inflammatory response (GO:0006954)integrin activation (GO:0033622)integrin binding (GO:0005178)leukocyte adhesive activation (GO:0050902)leukocyte chemotaxis (GO:0030595)leukocyte migration involved in inflammatory response (GO:0002523)membrane (GO:0016020)membrane (GO:0016020)microglial cell activation (GO:0001774)microglial cell proliferation (GO:0061518)negative regulation of apoptotic process (GO:0043066)negative regulation of apoptotic signaling pathway (GO:2001234)negative regulation of cell migration (GO:0030336)negative regulation of cell-substrate adhesion (GO:0010812)negative regulation of glutamate receptor signaling pathway (GO:1900450)negative regulation of hippocampal neuron apoptotic process (GO:0110091)negative regulation of interleukin-1 alpha production (GO:0032690)negative regulation of interleukin-1 beta production (GO:0032691)negative regulation of interleukin-6 production (GO:0032715)negative regulation of microglial cell activation (GO:1903979)negative regulation of neuron migration (GO:2001223)negative regulation of tumor necrosis factor production (GO:0032720)negative regulation of tumor necrosis factor production (GO:0032720)neuron cellular homeostasis (GO:0070050)neuron projection (GO:0043005)neuron remodeling (GO:0016322)neuronal cell body (GO:0043025)neuronal cell body (GO:0043025)perinuclear region of cytoplasm (GO:0048471)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive chemotaxis (GO:0050918)positive regulation of ERK1 and ERK2 cascade (GO:0070374)positive regulation of MAPK cascade (GO:0043410)positive regulation of actin filament bundle assembly (GO:0032233)positive regulation of calcium-independent cell-cell adhesion (GO:0051041)positive regulation of canonical NF-kappaB signal transduction (GO:0043123)positive regulation of canonical NF-kappaB signal transduction (GO:0043123)positive regulation of cell migration (GO:0030335)positive regulation of cell population proliferation (GO:0008284)positive regulation of cell-matrix adhesion (GO:0001954)positive regulation of inflammatory response (GO:0050729)positive regulation of microglial cell migration (GO:1904141)positive regulation of neuroblast proliferation (GO:0002052)positive regulation of neuron projection development (GO:0010976)positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction (GO:0051897)positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction (GO:0051897)positive regulation of release of sequestered calcium ion into cytosol (GO:0051281)positive regulation of smooth muscle cell proliferation (GO:0048661)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)regulation of lipopolysaccharide-mediated signaling pathway (GO:0031664)regulation of neurogenesis (GO:0050767)regulation of synaptic plasticity (GO:0048167)response to ischemia (GO:0002931)signaling receptor binding (GO:0005102)synapse pruning (GO:0098883)
Expression (TPM)
CX3CL1 — as a Regulated Gene

TFs regulating CX3CL1 0 TFs

Transcription factors with Perturb-seq knockdown data for CX3CL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CX3CL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to CX3CL1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CX3CL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr16:57,372,335–57,372,742 at TSS At TSS 581

Genome Browser

Genomic view of the CX3CL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr16:57,362,335 – 57,382,742
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq