Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]
Transcription factors with Perturb-seq knockdown data for CRYM. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CRYM upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CRYM, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:21,158,270–21,158,975 | 119.7 kb | Distal (>10kb) Multiome | 577 | |
| chr16:21,277,829–21,278,645 | 1 bp | At TSS Multiome | 636 | |
| chr16:21,283,543–21,284,106 | 5.5 kb | Proximal (<10kb) Multiome | 488 | |
| chr16:21,300,604–21,301,993 | 22.5 kb | Distal (>10kb) Multiome | 1062 | |
| chr16:21,302,514–21,303,208 | 24.5 kb | Distal (>10kb) Multiome | 136 | |
| chr16:21,501,368–21,502,964 | 224.0 kb | Distal (>10kb) Multiome | 766 | |
| chr16:21,519,749–21,521,002 | 242.2 kb | Distal (>10kb) Multiome | 859 | |
| chr16:21,521,389–21,522,043 | 243.5 kb | Distal (>10kb) Multiome | 479 | |
| chr16:22,436,881–22,437,622 | 1158.8 kb | Distal (>10kb) Multiome HiCAR | 642 |
Genomic view of the CRYM locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.