The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for CRNKL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CRNKL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CRNKL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:19,757,220–19,759,328 | 294.4 kb | Distal (>10kb) Multiome | 839 | |
| chr20:20,016,869–20,017,994 | 35.2 kb | Distal (>10kb) Multiome | 994 | |
| chr20:20,051,953–20,052,929 | 111 bp | At TSS Multiome | 945 | |
| chr20:20,102,068–20,103,125 | 50.4 kb | Distal (>10kb) Multiome | 37 | |
| chr20:20,134,692–20,135,494 | 82.6 kb | Distal (>10kb) Multiome | 124 |
Genomic view of the CRNKL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.