CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010]
Transcription factors with Perturb-seq knockdown data for CMBL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CMBL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CMBL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:10,101,981–10,102,627 | 205.6 kb | Distal (>10kb) Multiome | 152 | |
| chr5:10,102,752–10,103,804 | 204.5 kb | Distal (>10kb) Multiome | 152 | |
| chr5:10,156,936–10,157,799 | 150.5 kb | Distal (>10kb) Multiome | 159 | |
| chr5:10,249,033–10,250,873 | 57.8 kb | Distal (>10kb) Multiome | 961 | |
| chr5:10,307,259–10,308,125 | 106 bp | At TSS Multiome | 733 | |
| chr5:10,315,978–10,316,149 | 8.1 kb | Proximal (<10kb) | 39 | |
| chr5:10,332,886–10,333,985 | 25.7 kb | Distal (>10kb) Multiome | 288 | |
| chr5:10,352,751–10,354,487 | 45.8 kb | Distal (>10kb) Multiome | 1042 | |
| chr5:10,441,055–10,442,455 | 134.0 kb | Distal (>10kb) Multiome | 721 | |
| chr5:10,484,832–10,486,353 | 177.5 kb | Distal (>10kb) Multiome | 499 | |
| chr5:10,514,072–10,514,528 | 206.3 kb | Distal (>10kb) Multiome | 182 | |
| chr5:10,533,291–10,533,909 | 225.5 kb | Distal (>10kb) Multiome | 83 | |
| chr5:10,562,689–10,565,568 | 256.3 kb | Distal (>10kb) Multiome | 622 |
Genomic view of the CMBL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.