The product of this gene is a cytokine. Cytokines are small proteins that have an essential role in the immune and inflammatory responses. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. The protein encoded by this gene is a potent chemoattractant for neutrophils, monocytes and lymphocytes. It also can stimulate the proliferation of skeletal muscle cells. This protein may play important roles in inflammation and in the regeneration of skeletal muscle. Alternatively spliced transcript variants encoding different isoforms have been identified. Naturally occurring read-through transcription occurs between this locus and the neighboring locus CMTM1 (CKLF-like MARVEL transmembrane domain containing 1).[provided by RefSeq, Feb 2011]
Transcription factors with Perturb-seq knockdown data for CKLF. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CKLF upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CKLF, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:66,269,911–66,271,163 | 282.0 kb | Distal (>10kb) Multiome | 588 | |
| chr16:66,406,327–66,407,221 | 145.8 kb | Distal (>10kb) Multiome | 614 | |
| chr16:66,426,889–66,427,792 | 125.3 kb | Distal (>10kb) Multiome | 269 | |
| chr16:66,433,312–66,434,244 | 118.9 kb | Distal (>10kb) Multiome | 678 | |
| chr16:66,549,337–66,550,427 | 2.7 kb | Proximal (<10kb) Multiome | 817 | |
| chr16:66,552,056–66,553,030 | 3 bp | At TSS Multiome | 994 | |
| chr16:66,603,736–66,605,778 | 52.1 kb | Distal (>10kb) Multiome | 907 | |
| chr16:66,608,358–66,609,044 | 56.1 kb | Distal (>10kb) Multiome | 698 | |
| chr16:66,616,501–66,617,327 | 64.3 kb | Distal (>10kb) Multiome | 204 | |
| chr16:66,695,775–66,697,411 | 144.3 kb | Distal (>10kb) Multiome | 714 | |
| chr16:66,751,110–66,752,176 | 199.2 kb | Distal (>10kb) Multiome | 734 | |
| chr16:66,830,425–66,831,324 | 278.3 kb | Distal (>10kb) Multiome | 934 | |
| chr16:66,844,097–66,844,861 | 291.9 kb | Distal (>10kb) Multiome | 211 |
Genomic view of the CKLF locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.