The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. Alternatively spliced transcript variants differing only in their 5' UTRs have been found for this gene. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for CHST12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHST12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHST12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:2,232,261–2,233,424 | 170.7 kb | Distal (>10kb) Multiome | 809 | |
| chr7:2,241,746–2,242,506 | 161.4 kb | Distal (>10kb) Multiome | 794 | |
| chr7:2,313,875–2,314,819 | 89.1 kb | Distal (>10kb) Multiome | 684 | |
| chr7:2,353,760–2,355,437 | 48.9 kb | Distal (>10kb) Multiome | 1060 | |
| chr7:2,403,048–2,404,583 | 69 bp | At TSS Multiome | 775 | |
| chr7:2,451,319–2,452,043 | 48.0 kb | Distal (>10kb) Multiome | 660 | |
| chr7:2,518,679–2,520,557 | 116.1 kb | Distal (>10kb) Multiome | 567 | |
| chr7:2,555,131–2,556,097 | 152.2 kb | Distal (>10kb) Multiome | 772 | |
| chr7:2,558,641–2,559,572 | 155.4 kb | Distal (>10kb) Multiome | 669 | |
| chr7:2,631,170–2,632,859 | 228.2 kb | Distal (>10kb) Multiome | 658 | |
| chr7:2,646,545–2,647,390 | 243.5 kb | Distal (>10kb) Multiome | 245 | |
| chr7:2,688,023–2,688,723 | 284.7 kb | Distal (>10kb) Multiome | 176 |
Genomic view of the CHST12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.