This gene is found within a conserved gene cluster and encodes one of the beta subunits of the nicotinic acetylcholine receptor (nAChRs) superfamily which form ligand-gated ion channels with a central pore that forms a cation channel. Neuronal nAChRs are pentameric structures that can be either homomeric or heteromeric, with heteromeric structures containing both alpha and beta subunits. Each subunit contains an extracellular amino terminus and four transmembrane domains. Nicotine is one of the agonists that binds to the receptor. Variants in this gene have been associated with nicotine dependence and lung cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]
Transcription factors with Perturb-seq knockdown data for CHRNB4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHRNB4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHRNB4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:78,437,554–78,438,897 | 203.1 kb | Distal (>10kb) Multiome | 918 | |
| chr15:78,506,965–78,508,087 | 133.8 kb | Distal (>10kb) Multiome | 674 | |
| chr15:78,539,941–78,541,020 | 100.7 kb | Distal (>10kb) Multiome | 773 | |
| chr15:78,564,790–78,566,388 | 75.8 kb | Distal (>10kb) Multiome | 618 | |
| chr15:78,619,950–78,621,461 | 20.9 kb | Distal (>10kb) Multiome | 274 | |
| chr15:78,638,803–78,639,014 | 2.2 kb | Proximal (<10kb) | 150 | |
| chr15:78,640,579–78,641,869 | 121 bp | At TSS Multiome | 406 | |
| chr15:78,671,569–78,672,301 | 30.6 kb | Distal (>10kb) Multiome | 254 | |
| chr15:78,810,245–78,812,147 | 170.3 kb | Distal (>10kb) Multiome | 505 | |
| chr15:78,872,212–78,873,881 | 231.5 kb | Distal (>10kb) Multiome | 950 |
Genomic view of the CHRNB4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.