CHRNA3
cholinergic receptor nicotinic alpha 3 subunit

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]

Biological processes 66 terms
Golgi apparatus (GO:0005794)acetylcholine binding (GO:0042166)acetylcholine receptor activity (GO:0015464)acetylcholine receptor activity (GO:0015464)acetylcholine receptor signaling pathway (GO:0095500)acetylcholine receptor signaling pathway (GO:0095500)acetylcholine receptor signaling pathway (GO:0095500)acetylcholine-gated channel complex (GO:0005892)acetylcholine-gated channel complex (GO:0005892)acetylcholine-gated channel complex (GO:0005892)acetylcholine-gated channel complex (GO:0005892)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)behavioral response to nicotine (GO:0035095)calcium ion transport (GO:0006816)dendrite (GO:0030425)endoplasmic reticulum (GO:0005783)excitatory postsynaptic potential (GO:0060079)extracellular ligand-gated monoatomic ion channel activity (GO:0005230)ligand-gated monoatomic ion channel activity (GO:0015276)locomotory behavior (GO:0007626)membrane (GO:0016020)membrane (GO:0016020)membrane depolarization (GO:0051899)membrane depolarization (GO:0051899)monoatomic ion channel activity (GO:0005216)monoatomic ion transmembrane transport (GO:0034220)monoatomic ion transmembrane transport (GO:0034220)monoatomic ion transport (GO:0006811)monoatomic ion transport (GO:0006811)nervous system development (GO:0007399)neuromuscular synaptic transmission (GO:0007274)neuron projection (GO:0043005)neuronal cell body (GO:0043025)neurotransmitter receptor activity (GO:0030594)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane raft (GO:0044853)postsynaptic membrane (GO:0045211)presynaptic membrane (GO:0042734)presynaptic modulation of chemical synaptic transmission (GO:0099171)protein binding (GO:0005515)regulation of acetylcholine secretion, neurotransmission (GO:0014056)regulation of appetite (GO:0032098)regulation of dendrite morphogenesis (GO:0048814)regulation of membrane potential (GO:0042391)regulation of smooth muscle contraction (GO:0006940)response to acetylcholine (GO:1905144)response to nicotine (GO:0035094)signal transduction (GO:0007165)synapse (GO:0045202)synapse (GO:0045202)synapse (GO:0045202)synaptic membrane (GO:0097060)synaptic transmission involved in micturition (GO:0060084)synaptic transmission involved in micturition (GO:0060084)synaptic transmission, cholinergic (GO:0007271)synaptic transmission, cholinergic (GO:0007271)transmembrane signaling receptor activity (GO:0004888)
Expression (TPM)
CHRNA3 — as a Regulated Gene

TFs regulating CHRNA3 0 TFs

Transcription factors with Perturb-seq knockdown data for CHRNA3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHRNA3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to CHRNA3

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHRNA3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr15:78,340,100–78,340,745 280.7 kb Distal (>10kb) Multiome 194
chr15:78,437,554–78,438,897 182.8 kb Distal (>10kb) Multiome 918
chr15:78,506,965–78,508,087 113.5 kb Distal (>10kb) Multiome 674
chr15:78,539,941–78,541,020 80.5 kb Distal (>10kb) Multiome 773
chr15:78,564,790–78,566,388 55.5 kb Distal (>10kb) Multiome 618
chr15:78,618,487–78,618,677 2.3 kb Proximal (<10kb) 205
chr15:78,619,950–78,621,461 693 bp At TSS Multiome 274
chr15:78,640,579–78,641,869 20.4 kb Distal (>10kb) Multiome 406
chr15:78,671,569–78,672,301 50.8 kb Distal (>10kb) Multiome 254
chr15:78,810,245–78,812,147 190.6 kb Distal (>10kb) Multiome 505
chr15:78,872,212–78,873,881 251.8 kb Distal (>10kb) Multiome 950

Genome Browser

Genomic view of the CHRNA3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr15:78,330,100 – 78,883,881
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq