This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
Transcription factors with Perturb-seq knockdown data for CFL2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CFL2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CFL2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:34,461,634–34,463,144 | 252.2 kb | Distal (>10kb) Multiome | 931 | |
| chr14:34,629,461–34,630,514 | 84.4 kb | Distal (>10kb) Multiome | 907 | |
| chr14:34,693,166–34,693,989 | 21.2 kb | Distal (>10kb) Multiome | 463 | |
| chr14:34,713,982–34,715,044 | 47 bp | At TSS Multiome | 919 | |
| chr14:34,873,262–34,875,515 | 160.6 kb | Distal (>10kb) Multiome | 1146 | |
| chr14:34,981,885–34,983,443 | 268.1 kb | Distal (>10kb) Multiome | 1124 |
Genomic view of the CFL2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.