Predicted to enable calmodulin binding activity. Predicted to be involved in motile cilium assembly. Predicted to act upstream of or within epithelial cilium movement involved in extracellular fluid movement; establishment of localization in cell; and sperm flagellum assembly. Predicted to be located in axoneme and manchette. Predicted to be active in 9+2 motile cilium. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for CFAP221. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CFAP221 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CFAP221, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:119,366,351–119,367,938 | 177.5 kb | Distal (>10kb) Multiome | 994 | |
| chr2:119,431,298–119,432,803 | 112.7 kb | Distal (>10kb) Multiome | 903 | |
| chr2:119,476,117–119,476,795 | 67.9 kb | Distal (>10kb) Multiome | 482 | |
| chr2:119,523,786–119,524,663 | 20.1 kb | Distal (>10kb) Multiome | 187 | |
| chr2:119,537,405–119,538,283 | 6.6 kb | Proximal (<10kb) Multiome | 122 | |
| chr2:119,541,581–119,541,755 | 2.7 kb | Proximal (<10kb) | 12 | |
| chr2:119,544,074–119,545,082 | 34 bp | At TSS Multiome | 610 | |
| chr2:119,678,779–119,679,506 | 134.7 kb | Distal (>10kb) Multiome | 786 | |
| chr2:119,702,889–119,703,364 | 158.6 kb | Distal (>10kb) Multiome | 436 | |
| chr2:119,759,053–119,760,696 | 215.3 kb | Distal (>10kb) Multiome | 1033 |
Genomic view of the CFAP221 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.