This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1. [provided by RefSeq, May 2014]
Transcription factors with Perturb-seq knockdown data for CDCA4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CDCA4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CDCA4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:104,723,348–104,724,675 | 297.0 kb | Distal (>10kb) Multiome | 710 | |
| chr14:104,752,085–104,753,760 | 268.2 kb | Distal (>10kb) Multiome | 924 | |
| chr14:104,768,378–104,769,330 | 252.3 kb | Distal (>10kb) Multiome | 614 | |
| chr14:104,793,970–104,794,513 | 226.8 kb | Distal (>10kb) Multiome | 302 | |
| chr14:104,795,078–104,796,304 | 225.3 kb | Distal (>10kb) Multiome | 456 | |
| chr14:104,799,612–104,801,541 | 221.4 kb | Distal (>10kb) Multiome | 749 | |
| chr14:104,815,517–104,816,820 | 205.0 kb | Distal (>10kb) Multiome | 1064 | |
| chr14:104,825,631–104,828,095 | 193.3 kb | Distal (>10kb) Multiome | 653 | |
| chr14:104,863,847–104,866,207 | 156.8 kb | Distal (>10kb) Multiome | 743 | |
| chr14:104,932,604–104,933,678 | 87.6 kb | Distal (>10kb) Multiome | 498 | |
| chr14:104,967,607–104,968,024 | 53.2 kb | Distal (>10kb) Multiome | 196 | |
| chr14:104,975,803–104,976,561 | 44.9 kb | Distal (>10kb) Multiome | 394 | |
| chr14:104,977,531–104,979,147 | 42.6 kb | Distal (>10kb) Multiome | 387 | |
| chr14:104,985,398–104,986,377 | 35.3 kb | Distal (>10kb) Multiome | 518 | |
| chr14:105,020,271–105,022,072 | 159 bp | At TSS Multiome | 813 | |
| chr14:105,045,704–105,046,235 | 24.8 kb | Distal (>10kb) Multiome | 635 | |
| chr14:105,086,627–105,087,884 | 65.9 kb | Distal (>10kb) Multiome | 590 | |
| chr14:105,090,382–105,091,009 | 69.6 kb | Distal (>10kb) Multiome | 375 | |
| chr14:105,092,908–105,094,307 | 72.8 kb | Distal (>10kb) Multiome | 495 | |
| chr14:105,167,331–105,169,463 | 148.0 kb | Distal (>10kb) Multiome | 496 | |
| chr14:105,180,714–105,181,828 | 160.2 kb | Distal (>10kb) Multiome | 392 | |
| chr14:105,248,175–105,249,386 | 227.5 kb | Distal (>10kb) Multiome | 403 | |
| chr14:105,299,994–105,301,984 | 280.2 kb | Distal (>10kb) Multiome | 829 | |
| chr14:105,313,768–105,315,755 | 293.5 kb | Distal (>10kb) Multiome | 584 |
Genomic view of the CDCA4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.