Transcription factors with Perturb-seq knockdown data for CDC20-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CDC20-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CDC20-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:43,147,490–43,148,180 | 210.9 kb | Distal (>10kb) Multiome | 318 | |
| chr1:43,172,043–43,172,806 | 186.1 kb | Distal (>10kb) Multiome | 767 | |
| chr1:43,270,344–43,271,458 | 88.0 kb | Distal (>10kb) Multiome | 301 | |
| chr1:43,285,284–43,285,996 | 73.0 kb | Distal (>10kb) Multiome | 403 | |
| chr1:43,348,408–43,350,097 | 9.4 kb | Proximal (<10kb) Multiome | 819 | |
| chr1:43,358,112–43,359,560 | 148 bp | At TSS Multiome | 818 | |
| chr1:43,366,277–43,366,800 | 7.8 kb | Proximal (<10kb) Multiome | 226 | |
| chr1:43,366,988–43,368,619 | 9.4 kb | Proximal (<10kb) Multiome | 991 | |
| chr1:43,389,284–43,390,213 | 31.2 kb | Distal (>10kb) Multiome | 905 | |
| chr1:43,452,851–43,454,257 | 95.0 kb | Distal (>10kb) Multiome | 455 | |
| chr1:43,454,433–43,454,997 | 96.0 kb | Distal (>10kb) Multiome | 267 | |
| chr1:43,530,512–43,532,007 | 172.3 kb | Distal (>10kb) Multiome | 520 | |
| chr1:43,545,657–43,546,353 | 187.4 kb | Distal (>10kb) Multiome | 445 | |
| chr1:43,549,934–43,550,659 | 191.6 kb | Distal (>10kb) Multiome | 343 | |
| chr1:43,565,502–43,566,158 | 207.2 kb | Distal (>10kb) Multiome | 173 | |
| chr1:43,649,463–43,650,795 | 291.4 kb | Distal (>10kb) Multiome | 997 |
Genomic view of the CDC20-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.