Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CAPN5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CAPN5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CAPN5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:76,782,794–76,784,887 | 282.7 kb | Distal (>10kb) Multiome | 929 | |
| chr11:76,798,746–76,799,610 | 267.6 kb | Distal (>10kb) Multiome | 661 | |
| chr11:76,807,112–76,807,736 | 259.4 kb | Distal (>10kb) Multiome | 373 | |
| chr11:76,860,430–76,861,513 | 206.1 kb | Distal (>10kb) Multiome | 706 | |
| chr11:77,039,527–77,040,269 | 27.0 kb | Distal (>10kb) Multiome | 214 | |
| chr11:77,066,090–77,067,711 | 85 bp | At TSS Multiome | 664 | |
| chr11:77,091,010–77,091,507 | 24.3 kb | Distal (>10kb) Multiome | 344 | |
| chr11:77,126,985–77,127,575 | 60.4 kb | Distal (>10kb) Multiome | 458 | |
| chr11:77,147,671–77,148,349 | 81.0 kb | Distal (>10kb) Multiome | 172 | |
| chr11:77,171,758–77,172,392 | 105.1 kb | Distal (>10kb) Multiome | 275 | |
| chr11:77,191,860–77,192,374 | 125.2 kb | Distal (>10kb) Multiome | 447 |
Genomic view of the CAPN5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.