Transcription factors with Perturb-seq knockdown data for C8orf33. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C8orf33 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C8orf33, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:144,755,078–144,755,956 | 296.9 kb | Distal (>10kb) Multiome | 769 | |
| chr8:144,785,987–144,788,592 | 265.3 kb | Distal (>10kb) Multiome | 997 | |
| chr8:144,791,751–144,793,221 | 260.0 kb | Distal (>10kb) Multiome | 899 | |
| chr8:144,798,389–144,799,501 | 253.6 kb | Distal (>10kb) Multiome | 832 | |
| chr8:144,826,059–144,827,981 | 225.1 kb | Distal (>10kb) Multiome | 905 | |
| chr8:144,852,614–144,853,614 | 199.3 kb | Distal (>10kb) Multiome | 850 | |
| chr8:144,900,754–144,902,675 | 150.9 kb | Distal (>10kb) Multiome | 1021 | |
| chr8:144,950,374–144,951,024 | 101.7 kb | Distal (>10kb) Multiome | 695 | |
| chr8:145,002,551–145,003,548 | 49.5 kb | Distal (>10kb) Multiome | 872 | |
| chr8:145,051,576–145,052,751 | 128 bp | At TSS Multiome | 797 | |
| chr8:145,058,231–145,058,384 | 5.8 kb | Proximal (<10kb) | 22 | |
| chr8:145,058,539–145,058,804 | 6.1 kb | Proximal (<10kb) | 58 |
Genomic view of the C8orf33 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.