Transcription factors with Perturb-seq knockdown data for C4orf51. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C4orf51 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C4orf51, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:145,481,315–145,483,013 | 250.6 kb | Distal (>10kb) Multiome | 700 | |
| chr4:145,618,861–145,619,971 | 113.0 kb | Distal (>10kb) Multiome | 997 | |
| chr4:145,703,274–145,705,032 | 28.3 kb | Distal (>10kb) Multiome | 271 | |
| chr4:145,729,525–145,730,444 | 2.5 kb | Proximal (<10kb) Multiome | 46 | |
| chr4:145,732,348–145,733,906 | 653 bp | At TSS Multiome | 591 | |
| chr4:145,736,289–145,736,575 | 3.8 kb | Proximal (<10kb) | 119 | |
| chr4:145,887,216–145,887,983 | 155.1 kb | Distal (>10kb) Multiome | 199 | |
| chr4:145,909,209–145,909,939 | 177.1 kb | Distal (>10kb) Multiome | 176 | |
| chr4:145,935,719–145,938,595 | 203.5 kb | Distal (>10kb) Multiome | 694 | |
| chr4:145,938,677–145,939,163 | 206.5 kb | Distal (>10kb) Multiome | 664 |
Genomic view of the C4orf51 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.