Transcription factors with Perturb-seq knockdown data for C4orf33. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C4orf33 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C4orf33, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:128,802,742–128,803,879 | 290.7 kb | Distal (>10kb) Multiome | 154 | |
| chr4:128,809,374–128,810,275 | 284.0 kb | Distal (>10kb) Multiome | 764 | |
| chr4:128,810,520–128,812,873 | 281.5 kb | Distal (>10kb) Multiome | 1012 | |
| chr4:128,830,823–128,832,500 | 261.9 kb | Distal (>10kb) Multiome | 300 | |
| chr4:128,866,342–128,867,421 | 226.8 kb | Distal (>10kb) Multiome | 129 | |
| chr4:129,093,021–129,094,152 | 122 bp | At TSS Multiome | 827 | |
| chr4:129,096,037–129,096,647 | 2.3 kb | Proximal (<10kb) | 367 | |
| chr4:129,100,088–129,100,299 | 6.4 kb | Proximal (<10kb) | 2 | |
| chr4:129,164,187–129,164,904 | 70.7 kb | Distal (>10kb) Multiome | 85 | |
| chr4:129,205,784–129,206,798 | 112.7 kb | Distal (>10kb) Multiome | 121 |
Genomic view of the C4orf33 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.