Involved in negative regulation of ERK1 and ERK2 cascade and regulation of DNA-binding transcription factor activity. Located in extracellular space. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for C3orf33. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C3orf33 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C3orf33, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:155,744,096–155,745,472 | 62.0 kb | Distal (>10kb) Multiome | 726 | |
| chr3:155,745,739–155,746,446 | 60.1 kb | Distal (>10kb) Multiome | 309 | |
| chr3:155,805,529–155,806,648 | 52 bp | At TSS Multiome | 810 | |
| chr3:155,815,105–155,815,645 | 8.8 kb | Proximal (<10kb) | 51 | |
| chr3:155,853,753–155,855,235 | 48.3 kb | Distal (>10kb) Multiome | 1097 | |
| chr3:155,869,838–155,871,872 | 64.2 kb | Distal (>10kb) Multiome | 931 | |
| chr3:155,980,533–155,981,958 | 174.8 kb | Distal (>10kb) Multiome | 149 |
Genomic view of the C3orf33 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.